DNA sequencing III : dealing with difficult templates /
DNA sequencing III : dealing with difficult templates /
DNA sequencing three DNA sequencing 3
edited by Jan Kieleczawa.
- Sudbury, Mass. : Jones and Bartlett Publishers, c2009.
- xvi, 184 p., 8 p. of plates : ill. (some col.) ; 24 cm.
Includes bibliographical references and index.
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.
9780763742973 (alk. paper) 076374297X (alk. paper)
DNA--Analysis.
Nucleotide sequence--Methodology.
Sequence Analysis, DNA--methods.
Base Sequence.
Templates, Genetic.
572.86
Includes bibliographical references and index.
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.
9780763742973 (alk. paper) 076374297X (alk. paper)
DNA--Analysis.
Nucleotide sequence--Methodology.
Sequence Analysis, DNA--methods.
Base Sequence.
Templates, Genetic.
572.86