DNA sequencing III : dealing with difficult templates / edited by Jan Kieleczawa.
Material type: TextPublication details: Sudbury, Mass. : Jones and Bartlett Publishers, c2009.Description: xvi, 184 p., 8 p. of plates : ill. (some col.) ; 24 cmISBN:- 9780763742973 (alk. paper)
- 076374297X (alk. paper)
- DNA sequencing three
- DNA sequencing 3
- 572.86 22
Includes bibliographical references and index.
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.
Biotechnology & Bioinformatics