000			01629nam a2200289 a 4500
005			20230823095608.0
001			00013856
008			130616s2009 mauaf b 001 0 eng
020			_a9780763742973 (alk. paper)
020			_a076374297X (alk. paper)
040			_aDLC _cBD-DhIUB
082	0	0	_a572.86 _222
245	0	0	_aDNA sequencing III : _bdealing with difficult templates / _cedited by Jan Kieleczawa.
246	3		_aDNA sequencing three
246	3		_aDNA sequencing 3
260			_aSudbury, Mass. : _bJones and Bartlett Publishers, _cc2009.
300			_axvi, 184 p., 8 p. of plates : _bill. (some col.) ; _c24 cm.
504			_aIncludes bibliographical references and index.
505	0		_aControlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.
650		0	_aDNA _xAnalysis.
650		0	_aNucleotide sequence _xMethodology.
650	1	2	_aSequence Analysis, DNA _xmethods.
650	2	2	_aBase Sequence.
650	2	2	_aTemplates, Genetic.
700	1		_aKieleczawa, Jan.
999			_c13899 _d13899
526			_bbtbi