000 | 01629nam a2200289 a 4500 | ||
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005 | 20230823095608.0 | ||
001 | 00013856 | ||
008 | 130616s2009 mauaf b 001 0 eng | ||
020 | _a9780763742973 (alk. paper) | ||
020 | _a076374297X (alk. paper) | ||
040 |
_aDLC _cBD-DhIUB |
||
082 | 0 | 0 |
_a572.86 _222 |
245 | 0 | 0 |
_aDNA sequencing III : _bdealing with difficult templates / _cedited by Jan Kieleczawa. |
246 | 3 | _aDNA sequencing three | |
246 | 3 | _aDNA sequencing 3 | |
260 |
_aSudbury, Mass. : _bJones and Bartlett Publishers, _cc2009. |
||
300 |
_axvi, 184 p., 8 p. of plates : _bill. (some col.) ; _c24 cm. |
||
504 | _aIncludes bibliographical references and index. | ||
505 | 0 | _aControlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates. | |
650 | 0 |
_aDNA _xAnalysis. |
|
650 | 0 |
_aNucleotide sequence _xMethodology. |
|
650 | 1 | 2 |
_aSequence Analysis, DNA _xmethods. |
650 | 2 | 2 | _aBase Sequence. |
650 | 2 | 2 | _aTemplates, Genetic. |
700 | 1 | _aKieleczawa, Jan. | |
999 |
_c13903 _d13903 |
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526 | _bbtbi |