000			04180cam a2200649Ia 4500
001			ocn716174422
003			OCoLC
005			20230823095525.0
006			m o d
007			cr mn|||||||||
008			110428s2011 enkaf ob 001 0 eng d
040			_aUIU _beng _epn _cUIU _dDG1 _dYDXCP _dOSU _dCDX _dE7B _dN$T _dOCLCQ _dREDDC _dOCLCQ _dTEF _dUKMGB _dOCLCQ _dDEBSZ _dOCLCQ _dOCLCF _dOCLCQ
016	7		_a015638936 _2Uk
019			_a711780326 _a748937805
020			_a9780470669822 _q(electronic bk.)
020			_a0470669829 _q(electronic bk.)
020			_a9780470669815 _q(electronic bk.)
020			_a0470669810 _q(electronic bk.)
020			_z9780470975633
020			_z0470975636
020			_z9780470666586 _q(pbk.)
020			_z0470666587 _q(pbk.)
024	7	0	_a10.1002/9780470669815 _2doi
024	8		_a9786613239365
029	1		_aAU@ _b000047158723
029	1		_aDEBBG _bBV040076766
029	1		_aDEBBG _bBV043067297
029	1		_aDEBSZ _b372734863
029	1		_aDEBSZ _b421528796
029	1		_aNZ1 _b14256898
035			_a(OCoLC)716174422 _z(OCoLC)711780326 _z(OCoLC)748937805
050		4	_aQH585.2 _b.A565 2011eb
072		7	_aSCI _x017000 _2bisacsh
082	0	4	_a571.6/381 _222
049			_aMAIN
245	0	0	_aAnimal cell culture : _bessential methods / _ceditor, John M. Davis.
260			_aChichester, West Sussex ; _aHoboken, NJ : _bWiley-Blackwell, _c2011.
300			_a1 online resource (xxvii, 346 pages, [2] pages of color plates) : _billustrations (some color)
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
588	0		_aPrint version record.
504			_aIncludes bibliographical references and index.
520	3		_aAbstract: Animal cell culture is a widely used technology for producing recombinant proteins. The ability to make post-translational modifications and secrete the active forms of the protein into the culture medium represents major advantages over other processes. The growing market demand for pharmaceuticals has created a need for increased production capacity; however, achieving productivity gains in both the upstream stage and downstream processes can subject cells to aggressive environments such as those involving hydrodynamic stresses. Although numerous studies have explored the consequences of cell damage due to hydrodynamic stress, there has been a lack of understanding of the mechanism of such damage at a cellular level. Cell damage can also influence biomedical applications. Cells manipulated in instruments such as diagnosis and analysis devices can experience hydrodynamic forces.
505	0		_aThe cell culture laboratory / Sue Clarke and Janette Dillon -- Sterilization / Peter L. Roberts -- Microscopy of living cells / Colin Gray and Daniel Zicha -- Basic techniques and media, the maintenance of cell lines, and safety / John M. Davis -- Development and optimization of serum- and protein-free culture media / Stephen F. Gorfien and David W. Jayme -- Cryopreservation and banking of cell lines / Glyn N. Stacey, Ross Hawkins and Roland A. Fleck -- Primary culture of specific cell types and the establishment of cell lines / Kee Woei Ng [and others] -- Cloning / John Clarke, Alison Porter and John M. Davis -- The quality control of animal cell lines and the prevention, detection and cure of contamination / Peter Thraves and Cathy Rowe -- Systems for cell culture scale-up / Jennifer Halsall and John M. Davis -- Good laboratory practice in the cell culture laboratory / Barbara Orton.
650		0	_aCell culture _xTechnique.
650		0	_aTissue culture _xTechnique.
650		7	_aSCIENCE _xLife Sciences _xCell Biology. _2bisacsh
650		7	_aCell culture _xTechnique. _2fast _0(OCoLC)fst00850177
650		7	_aTissue culture _xTechnique. _2fast _0(OCoLC)fst01151483
655		4	_aElectronic books.
700	1		_aDavis, John, _d1952-
776	0	8	_iPrint version: _tAnimal cell culture. _dHoboken, NJ : John Wiley & Sons Inc., 2011 _z9780470666586 _w(DLC) 2010037008 _w(OCoLC)664450733
856	4	0	_uhttp://dx.doi.org/10.1002/9780470669815 _zWiley Online Library
994			_a92 _bDG1
999			_c18694 _d18653
526			_bls