| 000 | 05610cam a2200625Mi 4500 | ||
|---|---|---|---|
| 001 | ocn773564828 | ||
| 003 | OCoLC | ||
| 005 | 20230823095532.0 | ||
| 006 | m o d | ||
| 007 | cr |n|---||||| | ||
| 008 | 120123s2011 nju ob 000 0 eng d | ||
| 040 |
_aEBLCP _beng _epn _cEBLCP _dOCLCQ _dUIU _dN$T _dDG1 _dYDXCP _dOCLCQ _dOHI _dOCLCF _dUKDOC _dOCLCQ _dDEBSZ _dOCLCQ |
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| 019 | _a784138844 | ||
| 020 |
_a9783527644582 _q(electronic bk.) |
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| 020 |
_a352764458X _q(electronic bk.) |
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| 020 |
_a9783527644773 _q(electronic bk.) |
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| 020 |
_a3527644776 _q(electronic bk.) |
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| 029 | 1 |
_aAU@ _b000048852576 |
|
| 029 | 1 |
_aDEBSZ _b431074321 |
|
| 029 | 1 |
_aNZ1 _b15922069 |
|
| 029 | 1 |
_aDEBBG _bBV043394030 |
|
| 035 |
_a(OCoLC)773564828 _z(OCoLC)784138844 |
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| 037 |
_a10.1002/9783527644582 _bWiley InterScience _nhttp://www3.interscience.wiley.com |
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| 050 | 4 | _aQP624.5.D726 .H889 2011 | |
| 072 | 7 |
_aSCI _x029000 _2bisacsh |
|
| 082 | 0 | 4 | _a572.8633 |
| 049 | _aMAIN | ||
| 245 | 0 | 0 |
_aTag-based next generation sequencing / _cedited by Matthias Harbers and Günter Kahl. |
| 260 |
_aHoboken : _bJohn Wiley & Sons, _c2011. |
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| 300 | _a1 online resource (609 pages) | ||
| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 520 | _aTag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequ. | ||
| 505 | 0 | _aTag-based Next Generation Sequencing; Contents; Preface; List of Contributors; Part One: Tag-Based Nucleic Acid Analysis; 1 DeepSuperSAGE: High-Throughput Transcriptome Sequencing with Now- and Next-Generation Sequencing Technologies; 1.1 Introduction; 1.2 Overview of the Protocols; 1.2.1 Principle of the SuperSAGE Method; 1.2.2 Power of the SuperSAGE Tag; 1.2.3 Development of DeepSuperSAGE; 1.2.4 Ditag-Based DeepSuperSAGE (for 454 Pyrosequencing); 1.2.5 Single-Tag-Based DeepSuperSAGE (HT-SuperSAGE); 1.3 Methods and Protocols; 1.3.1 Linker or Adapter Preparation; 1.3.2 RNA Samples. | |
| 505 | 8 | _a1.3.3 cDNA Synthesis and NlaIII Digestion; 1.3.4 Tag Extraction from cDNA; 1.3.5 Tag Extraction from cDNA; 1.3.6 Purification of Linker-Tag Fragments; 1.3.7 Ditag or Adapter-Tag Formation and Amplification; 1.3.8 Preparation of Templates for Sequencing; 1.4 Applications; 1.4.1 Applications of DeepSuperSAGE in Combination with 454 Pyrosequencing; 1.4.2 Practical Analysis of HT-SuperSAGE; 1.5 Perspectives; References; 2 DeepCAGE: Genome-Wide Mapping of Transcription Start Sites; 2.1 Introduction; 2.2 What is CAGE?; 2.3 Why CAGE?; 2.4 Methods and Protocols; 2.4.1 Key Reagents and Consumables. | |
| 505 | 8 | _a2.4.2 Precautions; 2.4.3 RNA Samples Used for DeepCAGE Library Preparation; 2.4.4 DeepCAGE Library Preparation; 2.5 Applications; 2.6 Perspectives; References; 3 Definition of Promotome-Transcriptome Architecture Using CAGEscan; 3.1 Introduction; 3.2 What is CAGEscan?; 3.3 Why CAGEscan?; 3.4 Methods and Protocols; 3.4.1 Key Reagents and Consumables; 3.4.2 Precautions; 3.4.3 RNA Samples Used for CAGEscan Library Preparation; 3.4.4 Considerations on Pooling CAGEscan Libraries; 3.4.5 CAGEscan Library Preparation; 3.5 Applications and Perspectives; References. | |
| 505 | 8 | _a4 RACE: New Applications of an Old Method to Connect Exons; 4.1 Introduction; 4.2 Deep-RACE; 4.2.1 Choice of the Sequencer; 4.2.2 Validation of Promoter Studies; 4.2.3 Other Applications of Deep-RACE; 4.2.4 Limitations of Deep-RACE; 4.3 Methods Outline; 4.3.1 Primer Design; 4.3.2 Molecular Biology of Deep-RACE Library Preparation; 4.3.3 Sequencing of Deep-RACE Libraries; 4.3.4 Analysis; 4.4 Perspectives; References; 5 RNA-PET: Full-Length Transcript Analysis Using 5'- and 3'-Paired-End Tag Next-Generation Sequencing; 5.1 Introduction; 5.2 Methods and Protocols. | |
| 505 | 8 | _a5.2.1 Key Reagents and Consumables; 5.2.2 Protocol; 5.3 Applications; 5.3.1 PET Sequencing with SOLiD; 5.3.2 Mapping of the PETs; 5.3.3 PET Clustering, Annotation, and Genome Browser Visualization; 5.4 Perspectives; References; 6 Stranded RNA-Seq: Strand-Specific Shotgun Sequencing of RNA; 6.1 Introduction; 6.1.1 Before Starting; 6.2 Methods and Protocols; 6.2.1 Preface; 6.2.2 Materials and Consumables; 6.2.3 Protocol; 6.3 Bioinformatic Considerations; 6.4 Applications; 6.5 Perspectives; References. | |
| 505 | 8 | _a7 Differential RNA Sequencing (dRNA-Seq): Deep-Sequencing-Based Analysis of Primary Transcriptomes. | |
| 588 | 0 | _aPrint version record. | |
| 650 | 0 | _aDNA microarrays. | |
| 650 | 0 | _aNeurobiology. | |
| 650 | 4 | _aOligonucleotide array sequence analysis. | |
| 650 | 7 |
_aSCIENCE _xLife Sciences _xGenetics & Genomics. _2bisacsh |
|
| 650 | 7 |
_aDNA microarrays. _2fast _0(OCoLC)fst00886592 |
|
| 650 | 7 |
_aNeurobiology. _2fast _0(OCoLC)fst01036315 |
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| 655 | 4 | _aElectronic books. | |
| 700 | 1 | _aHarbers, Matthias. | |
| 700 | 1 | _aKahl, Günter. | |
| 776 | 0 | 8 |
_iPrint version: _tTag-based Next Generation Sequencing. _dHoboken : John Wiley & Sons, ©2011 _z9783527328192 |
| 856 | 4 | 0 |
_uhttp://dx.doi.org/10.1002/9783527644582 _zWiley Online Library |
| 994 |
_a92 _bDG1 |
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| 999 |
_c21413 _d21372 |
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| 526 | _bls | ||